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KMID : 0613820180280121448
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Journal of Life Science
2018 Volume.28 No. 12 p.1448 ~ p.1454
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Osteoblastogenic Activity of Locusta migratoria Ethanol Extracts on Pre-Osteoblastic MG-63 Cells
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Baek Min-Hee
Seo Min-Chul
Lee Joon-Ha
Kim In-Woo
Kim Mi-Ae
Hwang In-Yeong
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Abstract
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Insects have been investigated as a novel source of food and biomaterial in several recent studies. However, their osteoblastogenic cell activity has not been sufficiently researched and so, to investigate the potential of this natural material for promoting osteoblastogenesis, we studied the activity of Locusta migratoria ethanol extract (LME) on MG-63 pre-osteoblast cells. The cytotoxicity and proliferation effects of LME on MG-63 cells were measured by MTS assay, and there was no cytotoxicity up to 1,000 μg/ml. With LME treatment of 500 and 1,000 μg/ml for 48 hr, cell proliferation increased to 105% and 116% versus control, respectively. The osteoblastogenic activity of the LME was measured through alkaline phosphatase (ALP) staining at three and five days. As a result, both 500 and 1,000 μg/ml LME concentrations were seen to increase ALP activity by more than three times compared with control at three and five days. In addition, the expression level of the osteogenic markers ALP and RUNX2 was markedly increased after LME treatment. These results demonstrate that Locusta migratoria ethanol extract promotes osteoblastogenesis as evidenced by the increased osteogenic markers and suggest that LME may be a potential agent for bone formation and osteoporosis prevention.
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KEYWORD
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Alkaline phosphatase, bone formation, Locusta migratoria, MG-63, osteoblastogenesis
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